Selective cloning of hybridoma cells for enhanced immunoglobulin production using flow cytometric cell sorting and automated laser nephelometry
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چکیده
منابع مشابه
Using elicitors for enhanced production of secondary metabolites in plant cell and organ suspension cultures
In biotechnology has been concentrated to review alternative routes for the production of natural compounds. Plant cell culture systems are viable alternatives for the production of secondary metabolites that are of commercial importance in food and pharmaceutical industries. However, relatively very few cultures synthesize these compounds over extended periods in amounts comparable to those fo...
متن کاملUsing elicitors for enhanced production of secondary metabolites in plant cell and organ suspension cultures
In biotechnology has been concentrated to review alternative routes for the production of natural compounds. Plant cell culture systems are viable alternatives for the production of secondary metabolites that are of commercial importance in food and pharmaceutical industries. However, relatively very few cultures synthesize these compounds over extended periods in amounts comparable to those fo...
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Background: Invariant natural killer cells (iNKT) are an important immunoregulatory T cell subset. Currently several flow cytometry-based approaches exist for the identifi-cation of iNKT cells, which rely on using the 6B11 monoclonal antibody or a combina-tion of anti-Vα24 and anti-Vβ11 antibodies. Objective: The aim of this study was to compare the ability of two flow cytometry-based methods f...
متن کاملProspective isolation of multipotent pancreatic progenitors using flow-cytometric cell sorting.
During pancreatic development, neogenesis, and regeneration, stem cells might act as a central player to generate endocrine, acinar, and duct cells. Although these cells are well known as pancreatic stem cells (PSCs), indisputable proof of their existence has not been reported. Identification of phenotypic markers for PSCs leads to their prospective isolation and precise characterization to cle...
متن کاملIdentification and purification of classical Hodgkin cells from lymph nodes by flow cytometry and flow cytometric cell sorting.
We demonstrate the feasibility of using flow cytometry (FC) to identify the Hodgkin and Reed-Sternberg (HRS) cells of classical Hodgkin lymphoma (CHL). Initial flow cytometric studies of the HRS cell line L1236 demonstrated potentially useful antigens for identifying HRS cells. L1236 cells spontaneously bound normal T cells, analogous to the T-cell rosetting of HRS cells seen in tissue sections...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1990
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990110408